Reporter

Part:BBa_K2605000:Design

Designed by: Kaitlin Schaaf   Group: iGEM18_Calgary   (2018-10-09)


mCherry + BGH


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 979
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

A BmtI restriction site (sequence GCTAGC) was added to the 3' end of the BGH terminator so that this can be used as a reporter gene within our Multiple-Cloning Site flanked by FRT sites Part BBa_K2605002 system

Source

mCherry sequence was from part BBa_J176005

References